main product information
Other product information
Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser435 and Ser436 of human ATR (AA432-439)
Antibody's target
Human ATR (pSer435 + pSer436)
Antibody's full description
Rabbit Anti-Human ATR (pSer435 + pSer436) Polyclonal
Antibody's category
Polyclonal Antibodies
Antibody's other name
FRAP Related Protein 1 Antibody, MEC1 Antibody, RAC3 Antibody, SCKL Antibody
Verified applications
WB, AM
Raised in
Rabbit
Antibody's reactivity
Human
Antibody's recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
Antibody's purified from
Peptide Affinity Purified
Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
Antibody's concentration
See included datasheet or contact our support service
Antibody's specificity
Detects 301.367 kDa.
Storage recommendations
-20°C
Shipping recommendations
Blue Ice or 4°C
Certificate of analysis
A 1:250 dilution of SPC-918 was sufficient for detection of ATR (pSer435 + pSer436) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Antibody in cell
Nucleus, PML body
Tissue specificity
Ubiquitous
Scientific context
ATR is a protein-serine/threonine kinase. It is part of the PI3K family and functions in a complex with ATRIP. It responds to DNA damage and replication stresses and activates proteins that mediate DNA repair such as p53, BRCA1 and more. It other roles involve checkpoint signalling by interacting with chk1, fragile site stabilty and regulation of centrosome duplication.
Bibliography
Refer to PubMed
Released date
29/Aug/2016
NCBI number
NP_001175
Gene number
545
Protein number
Q13535
PubMed number
Refer to PubMed
Tested applications
To be tested
Tested reactivity
To be tested
Antibody's datasheet
Contact our support service to receive datasheet or other technical documentation.
Representative figure link
No Data Available
Warning information
Non-hazardous
Country of production
Canada
Total weight (kg)
1.4
Net weight (g)
0.1
Stock availabilit
In Stock
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Test
StressMark antibodies supplies antibodies that are for research of human proteins.
Properties
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Conjugation
Anti-FITC Antibody
Group
Polyclonals and antibodies
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name
Oryctolagus cuniculus