F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein
Atrogin-1 (Atr1) is also known as Muscle Atrophy F-box (MAFbx). It is an E3 ubiquitin ligase that initiates ATPdependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx is approximately a 43kDa protein.
Human, mouse, rat
Synthetic peptide surrounding amino acid 56 of rat Fbx32
100 µg (0.5 mg/ml) affinity purified rabbit anti-Fbx32 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide (Cat.# 3535BP-50) is available separately.
Shelf time: 12 months in temp.: -20 deg.C
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.