• Rabbit Anti-Human ATR (pSer435 + pSer436) Polyclonal: FITC

main product information

 
 

catalog no

SPC-918D-FITC

size

100 µl

price

389 EUR

Other product information

Immunogen

A phospho-specific peptide corresponding to residues surrounding Ser435 and Ser436 of human ATR (AA432-439)

Antibody's target

Human ATR (pSer435 + pSer436)

Antibody's full description

Rabbit Anti-Human ATR (pSer435 + pSer436) Polyclonal

Antibody's category

Polyclonal Antibodies

Antibody's other name

FRAP Related Protein 1 Antibody, MEC1 Antibody, RAC3 Antibody, SCKL Antibody

Verified applications

WB, AM

Raised in

Rabbit

Antibody's reactivity

Human

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Antibody's specificity

Detects 301.367 kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:250 dilution of SPC-918 was sufficient for detection of ATR (pSer435 + pSer436) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Nucleus, PML body

Tissue specificity

Ubiquitous

Scientific context

ATR is a protein-serine/threonine kinase. It is part of the PI3K family and functions in a complex with ATRIP. It responds to DNA damage and replication stresses and activates proteins that mediate DNA repair such as p53, BRCA1 and more. It other roles involve checkpoint signalling by interacting with chk1, fragile site stabilty and regulation of centrosome duplication.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

NCBI number

NP_001175

Gene number

545

Protein number

Q13535

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Properties

This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Conjugation

Anti-FITC Antibody

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus